Research Disclaimer: All compounds described are sold exclusively for in-vitro and laboratory research use. Not for unsupervised use. This content does not constitute medical advice.
When purchasing research compounds for laboratory use, the single most important quality indicator is an independent third-party Certificate of Analysis (COA) from a verified analytical laboratory. Janoshik Analytical (Prague, Czech Republic) has established itself as one of the most recognized third-party testing labs in the research compound space, providing HPLC-UV, HPLC-MS, and GC-MS testing with publicly verifiable results. Understanding how to read and interpret a Janoshik COA is essential for any researcher evaluating compound quality.
What Janoshik Tests and How#
Available analytical methods:
| Method | What it measures | Detection limit |
|---|---|---|
| HPLC-UV | Purity by area% (UV absorbance) | ~0.1% impurities |
| HPLC-MS/MS | Identity confirmation + purity | High specificity |
| GC-MS | Volatile compound identity/purity | Compound-dependent |
| NMR (H-1) | Structural confirmation | Full molecular structure |
| Elemental analysis | C, H, N, S percentages | Confirms molecular formula |
Sample submission: Researchers or suppliers submit a sample (typically 20–100 mg) directly to Janoshik. The lab tests it blind (without knowing claimed content) and issues the COA, which can be verified independently on the Janoshik website using the report ID.
Reading a COA: What to Check#
Key fields on a Janoshik COA:
-
Compound name and molecular formula: Confirms the correct compound was identified
-
CAS number: Cross-reference with published databases (PubChem, ChemSpider)
-
Method used: HPLC-UV (standard), HPLC-MS (more specific) — MS provides identity AND purity
-
Purity (area%): The percentage of the target compound relative to all detected peaks
- Research grade: ≥98% preferred; ≥95% acceptable for most research
- <95%: should prompt questions about the ~5% impurities
-
Retention time: Confirms compound matches library standard (identity check)
-
Date: COA should be recent (within 12–24 months); compounds degrade over time
What COA does NOT guarantee:
- Sterility (separate endotoxin/bioburden testing needed for in vivo use)
- Correct mass (COA tests concentration of a sample, not total mass in vial)
- Storage conditions post-testing (compound may have degraded since testing)
Why Third-Party vs. In-House Testing#
In-house "COA" (supplier self-tests):
- Supplier tests their own product → inherent conflict of interest
- Equipment quality and calibration unknown
- Methods may not be validated
- Results not independently verifiable
Third-party (Janoshik, Simec, Eurofins):
- Blind testing — lab doesn't know supplier's claimed values
- ISO/GLP-accredited methods in many cases
- Publicly verifiable: Janoshik report IDs searchable on their website
- Independent: no financial incentive to inflate results
The industry problem: Many suppliers claim "COA verified" but provide PDFs that cannot be verified. A genuine Janoshik COA has:
- A unique alphanumeric report ID
- Verifiable on janoshik.com → "Verify Report" search
- Lab name, address, analyst signature
JuicedPharma Testing Standard#
Every research compound sold by JuicedPharma is tested by independent third-party analytical laboratories including Janoshik. COA reports are available on each product page. We test:
- Every new compound batch before listing
- Peptide purity: HPLC-UV ≥98%
- Injectable compounds: HPLC-UV + identity (MS confirmation)
- Endotoxin: LAL test for all injectable compounds (≤10 EU/mg threshold)
Research Use Only. JuicedPharma does not provide medical advice.
Published by JuicedPharma Research Team · September 2026 · Category: Research Protocols & Science
